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eclipse ti e b wide field epifluorescence microscope  (Nikon)


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    Nikon eclipse ti e b wide field epifluorescence microscope
    Eclipse Ti E B Wide Field Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti+e+epifluorescence+microscope/Objectives/bio_rxiv__64898__2025__12__16__694648-266-11-16
    Average 99 stars, based on 59633 article reviews
    eclipse ti e b wide field epifluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Negative durotaxis: cell movement toward softer environments
    Article Snippet: .. Fabrication of steep stiffness gradients by controlled UV exposure Stiffness patterns were fabricated on photoresponsive hydrogels using a Nikon Eclipse Ti-E epifluorescence microscope and Plan Fluor 10x/0.30NA objective (Nikon), controlled by NIS-Elements software (Nikon). ..

    Article Title: Chemical LTP induces confinement of BDNF mRNA under dendritic spines and BDNF protein accumulation inside the spines
    Article Snippet: Neurons plated in IBIDI chamber slides (IBIDI GmbH, Germany) were transfected as described at point 2.1 with EX6-BDNFcds-12xMS2-3 L and MS2-NLS-mcherry or Ex1-BDNFcfds-12xMS2-3 L and MS2-NLS-mcherry, and time-lapse video images were taken 17–18 h after transfection. .. To acquire movies, a Nikon Eclipse Ti-E-epifluorescence microscope using a 40Xobjective (1.0 NA oil PlanApo DICH) equipped with a DS-Qi2 Camera (NIKON) was used. ..

    Article Title: Arhgef18 is a component of the outer limiting membrane required for retinal homeostasis
    Article Snippet: .. Images were captured with A Nikon Eclipse Ti-E epifluorescence microscope (using Nikon S Plan Fluor 20× (N.A., 0.45) or 60× oil (N.A., 1.4) objectives and NIS Elements Version 4.60 software) or a Leica SP8 confocal scanning microscope (63× (N.A., 1.40) oil objective and LAS X Version 3.5.7.23225 software). .. FFPE sections were used for haematoxylin and eosin staining (H&E) using a Leica ST 5010 Autostainer XL (Leica, Germany).

    Article Title: Druggable genome CRISPRi screen in 3D hydrogels reveals regulators of cortactin-driven actin remodeling in invading glioblastoma cells
    Article Snippet: .. Fluorescent images were taken on a Nikon Eclipse Ti-E epifluorescence microscope. ..

    Article Title: ZO-1 Regulates Hippo-Independent YAP Activity and Cell Proliferation via a GEF-H1- and TBK1-Regulated Signalling Network
    Article Snippet: Quantification of YAP nuclear translocation in ZO-1 siRNA experiments was performed using a pipeline of ImageJ/Fiji macros and a Python script. .. Briefly, cells grown on glass or polyacrylamide hydrogels were immunostained and imaged by acquiring multichannel z-stack images using the Nikon Eclipse Ti-E epifluorescence microscope with the 60× oil objective. ..

    Article Title: Druggable genome CRISPRi screen in 3D hydrogels reveals regulators of cortactin-driven actin remodeling in invading glioblastoma cells
    Article Snippet: .. Just prior to changing media, cell images were taken in 4x brightfield using a Nikon Eclipse Ti-E epifluorescence microscope Directly after imaging, media was removed and replaced with media containing 1.5 % v/v Poly-ethylene glycol (PEG) (TCI N0443). ..

    Article Title: ZO-1 Regulates Hippo-Independent YAP Activity and Cell Proliferation via a GEF-H1- and TBK1-Regulated Signalling Network
    Article Snippet: .. A Nikon Eclipse Ti-E epifluorescence microscope with a CFI Apochromat Nano-Crystal 60× oil objective (N.A., 1.2) (Nikon Europe, Amstelveen, The Netherlands) or a Leica TCS SP8 with an HC PL APO 40× (N.A., 1.30) or 63× (N.A., 1.40) oil objectives (Leica Microsystems, Wetzlar, Germany) were used for immunofluorescence imaging. .. Microscopy and immunoblot images were processed and adjusted using ImageJ/Fiji (Version 2.9.0) and Adobe Photoshop CC software (Version 2023, Adobe, San Jose, CA, USA).

    Article Title: Chemical LTP induces confinement of BDNF mRNA under dendritic spines and BDNF protein accumulation inside the spines
    Article Snippet: .. Once the immunostaining for c-fos was concluded, the images of neuronal cultures were acquired with a Nikon Eclipse Ti-E-epifluorescence microscope using a 20x objective. ..

    Software:

    Article Title: Negative durotaxis: cell movement toward softer environments
    Article Snippet: .. Fabrication of steep stiffness gradients by controlled UV exposure Stiffness patterns were fabricated on photoresponsive hydrogels using a Nikon Eclipse Ti-E epifluorescence microscope and Plan Fluor 10x/0.30NA objective (Nikon), controlled by NIS-Elements software (Nikon). ..

    Article Title: Arhgef18 is a component of the outer limiting membrane required for retinal homeostasis
    Article Snippet: .. Images were captured with A Nikon Eclipse Ti-E epifluorescence microscope (using Nikon S Plan Fluor 20× (N.A., 0.45) or 60× oil (N.A., 1.4) objectives and NIS Elements Version 4.60 software) or a Leica SP8 confocal scanning microscope (63× (N.A., 1.40) oil objective and LAS X Version 3.5.7.23225 software). .. FFPE sections were used for haematoxylin and eosin staining (H&E) using a Leica ST 5010 Autostainer XL (Leica, Germany).

    Imaging:

    Article Title: Druggable genome CRISPRi screen in 3D hydrogels reveals regulators of cortactin-driven actin remodeling in invading glioblastoma cells
    Article Snippet: .. Just prior to changing media, cell images were taken in 4x brightfield using a Nikon Eclipse Ti-E epifluorescence microscope Directly after imaging, media was removed and replaced with media containing 1.5 % v/v Poly-ethylene glycol (PEG) (TCI N0443). ..

    Article Title: ZO-1 Regulates Hippo-Independent YAP Activity and Cell Proliferation via a GEF-H1- and TBK1-Regulated Signalling Network
    Article Snippet: .. A Nikon Eclipse Ti-E epifluorescence microscope with a CFI Apochromat Nano-Crystal 60× oil objective (N.A., 1.2) (Nikon Europe, Amstelveen, The Netherlands) or a Leica TCS SP8 with an HC PL APO 40× (N.A., 1.30) or 63× (N.A., 1.40) oil objectives (Leica Microsystems, Wetzlar, Germany) were used for immunofluorescence imaging. .. Microscopy and immunoblot images were processed and adjusted using ImageJ/Fiji (Version 2.9.0) and Adobe Photoshop CC software (Version 2023, Adobe, San Jose, CA, USA).

    Immunofluorescence:

    Article Title: ZO-1 Regulates Hippo-Independent YAP Activity and Cell Proliferation via a GEF-H1- and TBK1-Regulated Signalling Network
    Article Snippet: .. A Nikon Eclipse Ti-E epifluorescence microscope with a CFI Apochromat Nano-Crystal 60× oil objective (N.A., 1.2) (Nikon Europe, Amstelveen, The Netherlands) or a Leica TCS SP8 with an HC PL APO 40× (N.A., 1.30) or 63× (N.A., 1.40) oil objectives (Leica Microsystems, Wetzlar, Germany) were used for immunofluorescence imaging. .. Microscopy and immunoblot images were processed and adjusted using ImageJ/Fiji (Version 2.9.0) and Adobe Photoshop CC software (Version 2023, Adobe, San Jose, CA, USA).

    Immunostaining:

    Article Title: Chemical LTP induces confinement of BDNF mRNA under dendritic spines and BDNF protein accumulation inside the spines
    Article Snippet: .. Once the immunostaining for c-fos was concluded, the images of neuronal cultures were acquired with a Nikon Eclipse Ti-E-epifluorescence microscope using a 20x objective. ..



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    Image Search Results


    Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E epifluorescence microscope (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.

    Journal: Journal of Neurochemistry

    Article Title: A Computational Approach to Identify Novel Protein Targets Uncovers New Potential Mechanisms of Action of Mirtazapine S (+) and R (−) Enantiomers in Rett Syndrome

    doi: 10.1111/jnc.70093

    Figure Lengend Snippet: Combined in vitro and in silico workflow for inverse docking target identification. (A) In vitro model to detect drug effects on Mecp2‐KO (RTT) neurons. Hippocampal primary cultures were seeded at DIV 0 in 96 Multi‐Well plates. After 3 days, Ara‐C and drug treatments were administered to the cells. At DIV 6, cells were fixed and immunofluorescence was performed to label dendrites and soma. Images were acquired at the Nikon Eclipse Ti‐E epifluorescence microscope (10x magnification objective) and each image was analyzed with the NeuriteQuant software. (B) Representative images for NeuriteQuant morphological analysis of Total Dendritic Length (TDL) and Endpoints (EPs) of DIV 6 hippocampal Mecp2 ‐KO neurons, plated at the density of 160 cells mm 2 . From left, Mecp2 ‐KO neurons treated with DMSO 0.1% (control condition), Amiloride and Felodipine at the concentration of 10 μM. (C, D) Treatments of neuronal cultures with 10 inactive molecules. Quantitative data of Mecp2 ‐KO neurons, reporting (C) the average TDL per neuron (fold change) and (D) the average number of EPs per neuron. n = 11 images for a total of 1 independent biological replicate (cell cultures). Error bars = mean ± SEM. Red line control condition normalized to = 1 (DMSO 0.1%). One‐way ANOVA (two‐tailed) with Dunnett's multiple comparisons test vs. DMSO conditions. ***p < 0.001, **p < 0.01, *p < 0.05. Grubbs' test was conducted to identify outliers. (E) In silico prediction method. First step: Download of R (−)MTZ and S (+)MTZ chemical structures from PubChem as SDF files; second step: BioGPS screening of R (−)/ S (+) MTZ versus 25 717 human pockets; third step: Comparison of affinity scores versus 10 inactive molecules. A one‐way ANOVA was performed to compare the effects of the ten drugs and the control (DMSO 0.1%). For TDL, F(10, 221) = 4.48, p ‐value = 0.0001. For EP, F(10, 221) = 4.41, p ‐value = 0.0001. Dunnett's multiple comparison test was then used to compare each drug to the control. Adjusted p ‐values for the TDL condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.58, Tubocuranine Cl (+) = 0.98, Alprenolol HCl = 0.99, Meglumine = 0.99, Piroxicam = 0.99, Amilopine = 0.54, Felodipine = 0.58, Pravadoline = 0.98. Adjusted p ‐values for the EP condition were: Spectinomycin = 0.99, Tizanidine HCl = 0.99, Galanthamine HBr = 0.74, Tubocuranine Cl (+) = 0.99, Alprenolol HCl = 0.99, Meglumine = 0.92, Piroxicam = 0.99, Amilopine = 0.38, Felodipine = 0.077, Pravadoline = 0.63.

    Article Snippet: Fluorescent images were captured using a Nikon Eclipse Ti‐E epifluorescence live imaging microscope, equipped with a Nikon DS‐Qi2 camera (CMOS sensor, 16.25 megapixel), and a 10X objective lens.

    Techniques: In Vitro, In Silico, Drug discovery, Immunofluorescence, Microscopy, Software, Control, Concentration Assay, Two Tailed Test, Comparison